Abstract

Objective. To study the antitumor mechanism of HOXB7 gene knockdown on the liver metastasis model of colon cancer in nude mice. Methods. Human colon cancer cell line HT29 transfected with pcdna3.1-hoxb7 knockdown or pcdna3.1-scrabble recombinant plasmid was inoculated into the spleen of nude mice to establish a stable liver metastasis model. There were 20 nude mice in the model, 10 of which were HOXB7 gene knockout mice and 10 of which were Scrabble mice. Four weeks after the model establishment, the histomorphology and histopathological changes of liver metastasis were observed. Results. The tumor formation rate of the liver metastasis model was 100% (20 / 20). Histomorphological analysis: compared with the control group, the study group significantly inhibited the formation of liver metastases in nude mice model; decreased or attenuated the size of liver metastases, liver mass, fusion between lesions, liver surface bulge and liver metastasis score, the difference was statistically significant (P < 0.05). Histopathological analysis: compared with the control group, the tumor cells in the study group were sparsely arranged, with marginal distribution, shallow infiltration depth, necrosis or apoptosis in the central area, the difference was statistically significant (P < 0.05). Conclusion. HOXB7 gene knock-out can effectively inhibit the formation of liver metastasis of colon cancer in nude mice, and the specific model of tumor inhibition and the establishment of quantitative standards need further study.

Abstract

Objective. To investigate the effect of FOLFOX 6 regimen on liver metastasis of colon cancer. Methods. 63 patients with newly diagnosed and unresectable colorectal cancer with liver metastasis were randomly divided into two groups. Group a received FOLFOX6 (oxaliplatin 85 mg / m2 and folic acid 200 mg / m2 intravenous infusion for 2 hours, followed by 5-FU 400 mg / m2 and 5-FU 2400 mg / m2 continuous infusion for 46 hours) chemotherapy, and repeated treatment every two weeks until the disease progressed or unacceptable toxicity appeared. Group B patients received oxaliplatin 130 mg / m2 intravenous infusion for 2 hours, day 1; calcium folinate 200 mg / m2 intravenous infusion for 2 hours, day 1-5, 5-FU 500 mg / m2 intravenous infusion for 4-6 hours, day 1-5; every 21 days is a cycle. The main end point was TTP. Kaplan Meier method was used to calculate the median survival time, and log rank method was used to compare the survival differences. Results. Among 63 patients, Cr was 3, PR 23, SD 13, PD 24, the effective rate was 41.3%. In group A, there were 3 cases of Cr, 17 cases of PR, 11 cases of SD and 10 cases of PD, with an effective rate of 48.8%; in group B, there were 0 cases of Cr, 6 cases of PR, 2 cases of SD and 14 cases of PD, with an effective rate of 27.3%. There was no significant difference between the two groups (χ 2 = 2.13, P > 0.05). The median survival time of 63 patients was 15 months, 21 months in group A and 13 months in group B; the median time to progress (TTP) was 9.8 months (range 0.5-31.8 months), 12 months in group A and 7 months in group B. the difference between the two groups was statistically significant (P = 0.002). The difference between the two groups was statistically significant (P = 0.023, P = 0.037). Conclusion. FOLFOX6 is an ideal clinical treatment for liver metastasis of colorectal cancer.

Abstract

Objective. Study the expression and significance of URP1 gene in lung cancer. Methods. Immunohistochemical SP method was used to detect the expression of URP1 in 152 lung cancer tissues and 152 matched normal lung tissues. Results. The expression of URP1 gene in lung cancer was significantly higher than that in normal lung tissue (P < 0.05), higher in non-small cell lung cancer and lower in small cell lung cancer, higher in squamous cell carcinoma (P < 0.05) than in adenocarcinoma (P < 0.05) and large cell carcinoma (P < 0.05), there was no significant difference between adenocarcinoma and large cell carcinoma (P > 0.05), and the expression level of URP1 gene was positively correlated with the differentiation degree of squamous cell carcinoma (P < 0.05). Conclusion. URP1 gene is involved in the occurrence and development of lung cancer.

Abstract

Objective. To study the pathological characteristics of borderline epithelial ovarian tumors and analyze the related factors of recurrence. Methods. From January 2011 to January 2013, 80 patients with borderline epithelial ovarian tumor (BOT) were selected to analyze their clinical data, diagnosis and treatment process and postoperative specimens, summarize their pathological characteristics, and analyze the factors affecting the recurrence. Results. The main clinical manifestations of 80 BOT patients were abdominal mass (77.50%). The histopathological types were serous tumor (26 cases), mucinous tumor (47 cases), and other types (7 cases). The pathological sections of 20 BOT patients showed that there was interstitial microinvasion or micropapillary, including non invasive implant (6 cases), peritoneum implant (11 cases), invasive implant (3 cases). Among 80 BOT patients, 6 cases recurred, all with interstitial microinvasion or microinvasion. Micropapillary patients. Conclusion. There are two types of pathological characteristics of borderline epithelial ovarian tumors: serous type and mucinous type. Some cases are accompanied with peritoneal implantation, interstitial microinvasion or papillary microgrowth. Interstitial microinvasion, peritoneal implantation and papillary microgrowth are the main factors affecting the recurrence of BOT. It is necessary to strengthen the postoperative observation and follow-up of patients with these pathological characteristics.

Abstract

Objective. Analyze the value of CYFRA21-1, CEA and NSE in the diagnosis of lung cancer. Methods. 40 lung cancer patients admitted to our hospital from March 2015 to June 2016 were selected as the observation group, and then 40 healthy people who came to our hospital for physical examination at the same time were selected as the control group. CYFRA21-1, CEA and NSE of the two groups were tested, and the relevant indicators were compared. Results. the levels of CYFRA21-1, CEA and NSE in the two groups were statistically significant (P < 0.05), and the levels of three markers in patients with squamous cell carcinoma, adenocarcinoma and small cell carcinoma were statistically significant (P < 0.05). Conclusion. CYFRA21-1, CEA and NSE have certain clinical value in the diagnosis of lung cancer, and can provide basis for the differentiation and diagnosis of lung cancer.

Abstract

Objective. Investigate the clinicopathological characteristics of epithelial tumors of the appendix which originated from appendicitis. Methods. The clinicopathological data of 15 cases of appendiceal epithelial tumors with appendicitis were analyzed retrospectively. Results. 15 cases of appendicitis, 6 were low grade appendiceal mucinous neoplasia (LaMn), the other 9 were prodromal lesions, including 8 serrated lesions and 1 villous tubular adenoma, of which 6 were sessile serrated adenoma / polyp (SSA / P) and 2 were traditional serrated glands. Tumor (TSA). 14 cases started with "acute appendicitis" and 1 case with "chronic appendicitis". Under the SSA / P microscope, the serrated structure and the expansion of the crypt were L or inverted T shape; the serrated outline and ectopic crypt were obvious in TSA, which had cell heteromorphism; the muscularis of the mucosa with serrated lesions was complete. LaMn was lined with slightly heterogeneous mucinous epithelium, with fibrosis or rupture of the tube wall. There was no cell mucinous pool in the tube wall and serosa. 9 cases were followed up, including 5 cases of prodromal lesions and 4 cases of LaMn, with a follow-up period of 1.0-81.5 months. Conclusion. Both serrated lesions and LaMn can be caused by acute appendicitis. In order to avoid pseudomyxoma of peritoneum caused by iatrogenic perforation, surgeons should improve their understanding of these diseases. The pathologist should take all the samples of this kind of appendiceal diseases for the purpose of differential diagnosis and report the condition of appendiceal margin.

Abstract

To investigate the value of serum sex hormone changes in patients with advanced lung adenocarcinoma after stereotactic radiotherapy (SBRT). Serum estradiol (E2), testosterone (T), follicle stimulating hormone (FSH), luteinizing hormone (LH) and progesterone (P) were measured in 198 untreated male patients with advanced lung adenocarcinoma and squamous cell carcinoma before and two months after SBRT. The levels of serum P, FSH and LH had no significant change before and after SBRT (P > 0.05), while the levels of E2 and t had significant difference after SBRT. Serum E2, t, P were significantly different before and after SBRT in the effective group (P < 0.05). According to pathological classification statistics, there were significant differences in serum E2, t and P in the effective treatment group before and after SBRT, while in the adenocarcinoma group, there were significant differences in serum E2, t and P before and after SBRT. Monitoring the changes of serum E2, t and P levels before and after SBRT has a certain value in predicting the curative effect of male advanced lung adenocarcinoma.

Abstract

Thymic epithelial tumor (TET) is relatively common in clinical work, especially since low-dose lung cancer screening, it has been found more and more. The biological behavior and pathological types of Tet are complex, and the degree of invasion of different types is different, which leads to differences in the formulation of clinical treatment strategies and prognosis evaluation. Therefore, it is of great significance to accurately predict the invasion and even pathological classification of Tet. A variety of imaging methods can find Tet, and each has its own advantages. This paper reviews the diagnostic value, especially the new progress.

Abstract

Objective. Investigate the effect of Smad2 / 3A on the development of neural crest cells in vertebrates. Methods. The expression of Smad2 / 3 gene was specifically knockdown by microinjection of Smad2 / 3 morpholine modified antisense oligonucleotides at the single cell stage of zebrafish embryo, and the expression of snail 1b, Sox10, FoxD3 and crestin were detected by whole embryo in situ hybridization. Results. Overexpression of Smad2 and smad3a were used, and the expression of crestin was also detected by whole embryo in situ hybridization. Results after Smad2 / 3A was knocked down, the expression of crestin decreased significantly, while the expression of snail 1b, Sox10 and FoxD3 did not change significantly. After smad3b was knocked down, there was no significant change in the expression of crestin, snail 1b, Sox10 and FoxD3; overexpression of casmad2 and smad3a could increase the expression of crestin; overexpression of casmad2 and smad3a could save the low expression of crestin caused by Smad2 / 3A knock down. Conclusion. Smad2 and smad3a play an important role in the expression of crestin.

Abstract

The study of cell adaptability of avian coronary bronchitis virus (IBV) is the basis of exploring the infection and transmission mechanism of IBV and producing IBV vaccine by using subculture cell bioreactor. It is the key to improve the production process and efficiency of IBV vaccine. In this paper, the history and current situation of cell adaptability, adaptive cell mechanism and sensitive cell receptor of IBV are reviewed, which will provide theoretical guidance for further study on the potential inter species transmission mechanism of IBV, the transformation or screening of cell adaptability and the development of cell adaptive virus vaccine.

Abstract

The heart consists of cells deriving from the cardiogenic plate and also from extracardiac sources. One of the major extracardiac contributions is given by the neural crest. The differentiation pathway and fate of the neural crest cells in the outflow tract have been followed over a prolonged period during outflow tract septation. We studied the role of the neural crest in remodeling the outflow tract by long-term cell tracing, differentiation markers and apoptosis.The pattern of neural crest cells migrating to the heart was investigated by heterospecific chicken quail chimeras and by retroviral infection of the reporter gene LacZ to the stemcells. The tagged neural crest cells move to areas that are morphogenetically active, such as the outflow tract, the semilunar valves, the wall of the arteries and the cardiac ganglia. Two differentiated subpopulations are discerned on the basis of immunohistochemical characterization with antibodies against smooth muscle cells in the arterial vessel wall and against ganglionic cells that were scattered around the vessels of the arterial pole and the heart. A third subpopulation did not stain with these antibodies, but presented locally with the phenomenon of apoptosis as shown with the TUNEL approach.In a developmental series of chicken embryos the populations were followed until stage 40. It was evident that the outflow tract septum in the early phase of development consisted mainly of mesenchymal neural crest cells. In a later phase neural crest cells were still detected at semilunar valve level, but nearly absent in the outflow tract septum below valve level. The septum at that time had become myocardialized. It is evident that neural crest cells are actually removed from this part of the heart by apoptosis. We are pursuing the hypothesis that an important function of apoptotic cells in heart development might be to activate the cardiomyocytes to muscularize the outflow tract septum through mobilizing or delivering growth factors at the time and place that septum formation is initiated.

Abstract

Objective. A Bombyx mori C / D box snoRNA bm-15 was found to interact with the notch like receptor gene (NLR) in vitro. The purpose of this study is to understand the interaction mechanism of snoRNA bm-15 and NLR in silkworm. Methods. The expression patterns of snoRNA bm-15 and NLR in the larva, pupa and adult of silkworm were detected by real-time fluorescence quantitative PCR, and the localization of snoRNA bm-15 and NLR in bmn4 cells was identified by in situ hybridization to explore the interaction mechanism between them. Results. The expression patterns of snoRNA bm-15 and NLR in the larval stage of silkworm were basically the same, showing a trend of first decreasing and then increasing, and the lowest expression level in the 2nd instar larvae. In the process of silkworm pupation, the expression of snoRNA bm-15 decreased gradually, and it was the lowest in the virgin moth. The expression of NLR was the lowest on the first day after pupation, and the highest in the virgin moth. Using in situ hybridization for cell localization, it was found that snoRNA bm-15 not only existed in bmn4 nucleus, but also in cytoplasm, while NLR mostly distributed in nucleus, overlapped with bm-15 in nucleus. Conclusion. For the first time, a snoRNA bm-15 was found in the nucleus and cytoplasm of Lepidoptera, and its interacting gene NLR mainly existed in the nucleus and overlapped with bm-15. There was the same expression pattern in larva stage of silkworm, and the opposite expression pattern in pupation and moth stage, suggesting that there might be different interaction patterns between snoRNA bm-15 and NLR in different development stages of silkworm.

Abstract

Objective. Investigate the effect of YB-1 gene silencing on chemotherapeutic sensitivity of hepatocarcinoma and its possible mechanism. Methods. According to YB-1 target sequence, YB-1 gene siRNA interference sequence was designed, linked with the linearized plkd-cmv-g & pr-u6 lentivirus vector after double enzyme digestion of eco R Ⅰ and bam h Ⅰ, and transformed into recombinant lentivirus; referring to this method, negative control recombinant lentivirus was designed and constructed, and then transfected into hepatoma cell SMMC-7721, named yb-1-sirna group and NC siRNA group respectively, and set up blank control group (without any treatment). SMMC-7721 cells were treated with cisplatin (1 μ g / ml) and doxorubicin (1 μ g / ml). The expression of YB-1 mRNA was detected by real-time fluorescence quantitative PCR, the proliferation of cells was detected by MTT method, the apoptosis of cells was detected by flow cytometry, and the expression of PI3K, Akt and GSK-3 β protein was detected by Western blot. Results. Before and after treatment, YB-1 mRNA expression level and cell viability of yb-1-sirna group, nc-sirna group and blank control group were significantly different (P < 0.05). Compared with before treatment, YB-1 mRNA expression level and cell viability of yb-1-sirna group, nc-sirna group and blank control group were significantly decreased (P < 0.05). Before and after treatment, the apoptosis rate of yb-1-sirna group, nc-sirna group and blank control group was significantly different (P < 0.05); compared with before treatment, the apoptosis rate of each group after treatment was significantly higher (P < 0.05). After drug treatment, there were significant differences in PI3K, Akt and GSK-3 β protein expression among the three groups (P < 0.05). Conclusion. Targeted knockout of YB-1 gene combined with chemotherapy can significantly enhance the ability of inhibiting proliferation and promoting apoptosis of hepatoma cells, which may be related to PI3K / Akt / GSK3 β signal transduction pathway.

Abstract

Objective. Investigate the molecular mechanism of IL-11 secretion by U2OS cells induced by hypoxia. Methods. human osteosarcoma U2OS cell line was cultured in hypoxia (1% O2) environment in vitro, and incubated with HIF-1 α inhibitor, or pre treated with different signal pathway inhibitors. Western blot was used to detect the protein level of HIF-1 α, quantitative PCR was used to detect the mRNA level of IL-11, and ELISA was used to detect the protein level of IL-11. Results. Compared with U2OS cells cultured in normal oxygen environment, hypoxia significantly induced the expression of HIF-1 α in U2OS cells, and increased the mRNA and protein levels of IL-11 in a time-dependent manner; the addition of HIF-1 α inhibitor significantly inhibited the expression of HIF-1 α and the mRNA and protein levels of IL-11; the addition of JNK signal inhibitor also significantly inhibited the expression and protein levels of IL-11 induced by hypoxia. The difference was statistically significant (P < 0.05). However, the expression of HIF-1 α had no significant difference (P > 0.05). Conclusion. Hypoxia can induce osteosarcoma U2OS cells to secrete IL-11 through HIF-1 α - JNK signal, thus promoting the development of osteosarcoma.

Abstract

To investigate the molecular mechanism of IL-11 secretion by U2OS cells induced by hypoxia. Human osteosarcoma U2OS cell line was cultured in hypoxia (1% O2) environment in vitro, and incubated with HIF-1 α inhibitor, or pre treated with different signal pathway inhibitors. Western blot was used to detect the protein level of HIF-1 α, quantitative PCR was used to detect the mRNA level of IL-11, and ELISA was used to detect the protein level of IL-11. Results compared with U2OS cells cultured in normal oxygen environment, hypoxia significantly induced the expression of HIF-1 α in U2OS cells, and increased the mRNA and protein levels of IL-11 in a time-dependent manner; the addition of HIF-1 α inhibitor significantly inhibited the expression of HIF-1 α and the mRNA and protein levels of IL-11; the addition of JNK signal inhibitor also significantly inhibited the expression and protein levels of IL-11 induced by hypoxia. The difference was statistically significant (P < 0.05). However, the expression of HIF-1 α had no significant difference (P > 0.05). Conclusion hypoxia can induce osteosarcoma U2OS cells to secrete IL-11 through HIF-1 α - JNK signal, thus promoting the development of osteosarcoma.

Abstract

Objective. To determine the inhibitory effect of 5-ALA-PDT on human skin squamous cell carcinoma A431 cells. Methods. the cells were incubated with 5-ALA and A431 cells. After being irradiated by 630 NLN red light, the inhibition rate of cell growth was detected by MTF method, and the inhibition effect of culture time, 5-ALA concentration and light dose on skin squamous cell carcinoma A431 cells was determined. Results. when the dose of light was 80 J / cm ~ 2 and the concentration of 5-ALA was 3.2 mmol / L, the inhibition rate was the highest when 5-ALA and A431 cells were incubated together for 120 min. Conclusion. 5-ALA-PDT can inhibit the proliferation of A431 cells in vitro.

Abstract

Objective. To investigate the effect of bisphenol A (BPA) on the proliferation and expression of MIR-19 in PC-3 cells. Methods. Human prostate cancer PC-3 cells were treated with different concentrations of BPA, and estradiol (E2) was used as the positive control. Six days later, MTT and Hoechst 33258 fluorescent staining were used to detect the effect of BPA on PC-3 cell proliferation. Real time PCR was used to detect the changes of mir-19a and mir-9b, Western blot was used to detect the target gene PTEN and cell proliferation related genes p-Akt and Akt of MIR-19. , PCNA and cyclin D1. Results. 5 × 10-6 ~ 5 × 10-5 mol / L BPA significantly promoted the proliferation of PC-3 cells, increased the expression of mir19a and mir19b in PC-3 cells, decreased the expression of PTEN, and increased the expression of p-Akt, PCNA and cyclin D1. Conclusion. BPA can promote the proliferation of PC-3 cells, which may be related to the upregulation of MIR-19 expression induced by BPA.

Abstract

Detect the expression of ERCC1 in patients with acute ischemic stroke (CIS) and to explore the relationship between ERCC1 and CIS. Methods peripheral blood samples and clinical data of 64 patients with acute CIS (stroke group) and 70 healthy people (control group) were collected. The relative expression level of ERCC1 mRNA in PBMCs was detected by real-time fluorescence quantitative PCR, and the expression level of ERCC1 protein in plasma was detected by ELISA. Results the expression level of ERCC1 mRNA in stroke group was significantly lower than that in control group (P < 0.05), and the expression level of ERCC1 protein was significantly higher than that in control group (P < 0.05). There was no significant difference in mRNA and protein expression of ERCC1 gene among different stroke types (P > 0.05). The expression level of ERCC1 gene mRNA was negatively correlated with the history of stroke (r = -0.409, P = 0.013), and there was no significant correlation between ERCC1 gene expression and the history of stroke (P > 0.05). Conclusion ERCC1 gene may be involved in the occurrence, development, prognosis and recurrence of acute CIS.

Abstract

Objective. To investigate the effect of overexpression of Notch 1 intracellular domain (NiCd) gene on the proliferation of human periodontal ligament stem cells (PDLSCs). Methods. retroviral particles containing over expression of NiCd gene were constructed and transfected into periodontal ligament stem cells. PDLSCs / NiCd cell line with over expression of NiCd was constructed. The general state of cells was observed 48 hours after transfection. PDLSCs / NiCd was used as the experimental group, PDLSCs / wt as the normal cell group and PDLSCs / vector empty virus transfection group as the control group. The expression of NiCd mRNA and protein was detected by quantitative real-time polymerase chain reaction (QRT PCR) and Western blot. Cell counting kit-8 (CCK-8) was used to detect the proliferation of cells. Cell migration was measured. Results. Western blot and qRT-PCR showed that compared with PDLSCs / wt group and PDLSCs / vector group, the expression level of NiCd protein and its mRNA in PDLSCs / NiCd group were significantly higher (P < 0.05); CCK-8 results showed that compared with PDLSCs / vector group, the proliferation of cells in PDLSCs / NiCd group was faster, and the cell migration (40.20 ± 1.74) in PDLSCs / NiCd group was significantly higher than that in PDLSCs / vector group. (21.20 ± 1.18). Conclusion. The proliferation and migration of human PDLSCs overexpressed by NiCd gene are enhanced to some extent.

Abstract

Cell-free DNA from the blood has permitted noninvasive tumor genome analysis. Meanwhile, mtDNA variations are associated with cancer pathogenesis. Since peritonealcarcinomatosis accompanied by malignant ascites is a major cause of death of advanced gastric cancer, this study aimed to characterize mtDNA variations from patients with gastric carcinoma, as well as the cfDNA variations in ascites. Method & Result. A meta-analysis showed that the mtDNA variations are associated with gastric carcinoma, especially in D-loop area (OR =3.34; 95 % CI 1.07–10.48; I2=77.8 %). UPGMA clustering analysis proved that the gastric cancer cell lines had a far genetic distance from hepatic cancer and colon cancer. In ascites from gastric carcinoma (n=32) and matched non-tumoral ones, the percentage of cell-free T152C and T16519C were significant in gastric cancer group (Both P'<'0.05). However, they were not related with clinicopathologic features among gastric cancers. Conclusion. MtDNA variations are associated with gastric cancer.

Abstract

To explore the pathogenic genes and key pathways of neutrophils in sepsis. The data of gse6535, gse49755, gse49756 and gse49757 were downloaded from the gene expression assembly (GEO) database. The differential expression gene (DEG) of neutrophils was identified by R software. Through the intersection of the two, a total of 93 DEG's were obtained, and further gene function and path enrichment analysis, PPI network analysis and key gene analysis were carried out by using David, string and the reactionfiplutin and cyclohubba applications in Cytoscape. The most important key DEG of neutrophils were identified, including toll like receptor 2 (TLR2), proto oncogene SRC, matrix metalloproteinase 9 (MMP9), interleukin-1 receptor 2 (il1r2), inhibitory protein β 1 (arrb1), interleukin-1 receptor related kinase 3 (irak3), interleukin-18 receptor 1 (il18r1), interleukin-18 receptor related protein (il18rap), serine / threonine kinase 17b (stk17b). We found some pathways related to sepsis and the biological processes related to immunity. These genes may cause sepsis through multiple signaling pathways.

Abstract

Syncytiotrophoblast (STB) is a kind of multinucleated epithelial cells, which is located in the outermost layer of human placenta. It is derived from the differentiation and fusion of cytotrophoblast (CTB). STB is responsible for the secretion of key hormones in pregnancy and the exchange of nutrients between mother and fetus. It is also an important part of the maternal fetal barrier. However, the regulatory mechanism of syncytial process of placental trophoblasts is still unclear. In this study, bioinformatics method was used to screen and preliminarily verify the key genes that potentially regulate the syncytial regulation of choriocarcinoma bevo cells. The microarray data sets of BeWo cells before and after the integration were retrieved and downloaded from the geo database, and all relevant data sets were analyzed comprehensively by bioinformatics analysis method. The differentially expressed genes were screened and analyzed by go and KEGG cluster analysis. Further, the node genes were searched by PPI egg white interaction network. Finally, the differential expression of candidate genes before and after the integration was verified by qRT-PCR. A total of 137 differentially expressed genes were screened from the two sets of data, 25 of which were shared by the two sets of data, and were involved in cell migration, cell adhesion, hormone metabolism, MAPK signaling pathway, etc. Further, three candidate genes were obtained by protein interaction network analysis, which were verified by QRT PCR, and the results were consistent with the information analysis results. This study provides a potential target gene for syncytial study of trophoblast and a clue for the analysis of its molecular regulatory mechanism.

Abstract

Objective. To study the gene expression profile of breast cancer by oligonucleotide microarray. Methods. The target gene was obtained according to the literature, the corresponding gene mRNA sequence was inquired, the probe was designed and synthesized by computer, and the probe was spot sampled on the chemically modified slide to prepare the tumor related gene oligonucleotide chip containing 288 genes. The total RNA of breast cancer and corresponding normal tissues was extracted, and then the cDNA probe with fluorescent label was reverse recorded and hybridized with the chip. Results. There were significant differences in gene expression between 16 cases of breast cancer and normal breast tissues, including 6 kinds of high expression and 4 kinds of low expression. Conclusion. There are some differentially expressed genes in breast cancer and normal breast tissue, and the occurrence and development of breast cancer are closely related to these genes. Oligonucleotide microarray technology is of great significance in the research of breast cancer related genes.

Abstract

Objective. To investigate the effect of siRNA interference with S100A4 Protein on human gastric cancer cells. Methods. sirna-nc and sirna-s100a4 were transfected into SGC-7901 and MGC-803 by lip2000. The change of S100A4 gene expression was verified by qRT-PCR, and the change of cell proliferation was detected by MTT, cell clone and apoptosis. The changes of invasion and migration ability were detected by Transwell test and cell scratch test, and intracellular calcium concentration was measured by fura-2 acetylcarboxymethyl (fura-2 / AM). Western blot was used to detect the expression of PKC - α, cam and S100A4. Results. compared with the sirna-nc group, the level of S100A4 gene in the sirna-s100a4 group was significantly reduced; the ability of cell proliferation, invasion and migration was also reduced; the apoptosis of cells in the sirna-s100a4 group was increased, and the concentration of PKC - α, cam, S100A4 protein and free calcium ion in the cells were decreased. Conclusion. the down-regulation of S100A4 results in the decrease of intracellular calcium concentration, which leads to the decrease of PKC - α and cam protein expression which need calcium activation to play a role, and then induces a series of biological function changes.

Abstract

Objective: To investigate the differentially expressed genes and their biological significance in lymph node metastasis of early gastric cancer by using RNA SEQ data of TCGA database. Methods: to download RNA SEQ data and clinicopathological data of early gastric cancer from TCGA database, screen out data of early gastric cancer, and make a comparative analysis according to whether there is lymph node metastasis. In r3.5.3 environment, load edger package, and screen T1 n1-3 M0 and t. Results: 197 up-regulated genes and 5 down-regulated genes were found in early gastric cancer with lymph node metastasis. Conclusion: the differentially expressed genes in lymph node metastasis of early gastric cancer may be related to the prognosis of patients with gastric cancer. The biological behavior of early gastric cancer is influenced by the signal pathways of cell proliferation related go function, Wnt signal pathway and NF - κ B signal pathway. The differential expression level of genes in early gastric cancer with and without lymph nodes is significantly related to the prognosis of patients with gastric cancer, which may be used as the diagnosis and treatment target of gastric cancer.

Abstract

silver ions were added in the process of microbial leaching of cobalt ores. The effects of silver ions on the growth of bacteria and bioleaching behavior of cobalt ores were investigated. The results showed that the amount of silver ions had a direct impact on the growth of bacteria. When the amount of silver ions was less than 20 mg / L, the growth of bacteria was not affected by silver ions, but the growth of bacteria would be affected by increasing the concentration of silver ions. The addition of silver ion can accelerate the oxidation dissolution rate of cobalt bearing minerals and significantly improve the metal leaching rate. Under the conditions of pulp concentration of 10%, leaching temperature of 38 ℃, rotating speed of 160 R / min and silver ion concentration of 15 mg / L, the catalytic effect of silver ion is the best. At this time, the leaching rate of cobalt and copper can be increased by 28.0% and 26.8%.

Abstract

To investigate the role and molecular mechanism of azd8055, a dual inhibitor of mammalian rapamycin target protein (mTOR), in inhibiting the migration and EMT process of human cholangiocarcinoma cell line hucct1. Methods. MTT assay and plate clone formation assay were used to detect the effect of azd8055 on the proliferation of cholangiocarcinoma cells. Scratch healing assay and Transwell cell migration assay were used to detect the effect of azd8055 on the migration of hucct1 cells. Western blot was used to detect the expression of EMT marker related protein, Akt / mTOR signaling pathway protein and DEK protein. The interaction of azd8055, DEK and Akt signaling pathway was analyzed by using stitch and genemania database. After DEK gene silencing, the proliferation activity, migration ability and Akt / mTOR signaling pathway related protein expression level of cholangiocarcinoma cells were detected. Results. Azd8055 could inhibit the expression of cholangiocarcinoma cells. Conclusion. Azd8055 can inhibit the migration and EMT of hucct1 cells by down regulating the DEK and Akt / mTOR signaling pathway.

Abstract

Objective. To investigate the expression level and clinical significance of Podoplanin and VEGF-C in gastric cancer. Methods. 127 patients with gastric cancer were treated with paraffin. The expression level of Podoplanin and VEGF-C was detected by immunohistochemistry. Results the relationship between Podoplanin and VEGF-C and clinicopathological parameters was observed. The expression level of Podoplanin and VEGF-C in the tissues was higher than that in the adjacent tissues (P < 0.05). There was no significant difference in the expression level of Podoplanin in the tissues of gastric cancer patients with different age, gender and tumor size (P > 0.05). The expression level of Podoplanin was higher in the patients with high clinical stage, low degree of differentiation, serous infiltration and lymph node metastasis (P < 0.05). There was no significant difference in the expression rate of VEGF-C in the cancerous tissues of gastric cancer patients with chemokines (P > 0.05). The expression rate of VEGF-C was higher in the patients with high clinical stage, serous infiltration and lymph node metastasis (P < 0.05). Conclusion the expression level of Podoplanin and VEGF-C was higher in the cancerous tissues of gastric cancer. The high expression of Podoplanin may be related to the clinical stage, differentiation degree, serous infiltration and lymph node metastasis. The positive expression rate of VEGF - C may be related to clinical stage, serous infiltration and lymph node metastasis.

Abstract

Objective. To clone the protein coding region of Pxn gene and construct an eukaryotic expression vector of Pxn gene, establish a Pxn stable transformed hepatoma cell line, and analyze the effect of Pxn gene on hepatoma cells. Methods. Total RNA of hepatoma cells was extracted by Trizol method, and then reverse transcripted into cDNA. The Pxn gene coding region was amplified by PCR and eukaryotic expression plasmid pebfp-pxn was constructed. HepG2 and G418 were transfected by liposome method. Results. Pxn gene was successfully cloned and eukaryotic expression vector pebfp-pxn was constructed. The cell line with high expression of pegylated protein was obtained. The cell growth rate was detected by real-time tagless cell analysis, and the cell migration rate was analyzed by cell trauma healing experiment. The protein expression was detected by Western blotting. The expression of bfp-pxn did not affect the growth rate of HepG2 hepatoma cells, but could inhibit the migration of HepG2 hepatoma cells. The expression of E-cadherin and N-cadherin and vimentin decreased. Conclusion. Pxn gene did not affect the growth and proliferation rate of HepG2 hepatoma cells, but could inhibit the migration of HepG2 hepatoma cells. Cell migration may be related to its effect on the expression of epithelial - stromal transformation marker protein.